Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination

27Citations
Citations of this article
39Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

In this article, a novel amperometric acetylcholine (ACh) biosensor with immobilization of acetylcholinesterase–choline oxidase (AChE–ChO) on electrochemically polymerized polyaniline–polyvinylsulphonate (PANI–PVS) film has been successfully developed for the first time. AChE and ChO enzymes were immobilized on PANI–PVS film by cross-linking with using glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of ACh was carried out by oxidation of enzymatically produced H2O2 at +0.4 V versus Ag/AgCl. Optimum pH and temperature were found to be 8.0 and 60 °C, respectively. The linear part was in the region 1.0 × 10−7–6.0 × 10−7 M (R2 = 0.990). Storage stability and operation stability of the enzyme electrode were also studied.

Cite

CITATION STYLE

APA

Tunç, A. T., Aynacı Koyuncu, E., & Arslan, F. (2016). Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination. Artificial Cells, Nanomedicine and Biotechnology, 44(7), 1659–1664. https://doi.org/10.3109/21691401.2015.1080167

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free