Direct Electron Transfer of Hemoglobin on Manganese III Oxide-Ag Nanofibers Modified Glassy Carbon Electrode

Abstract

We investigated the electrochemical behavior of hemoglobin by glassy carbon electrode modified with Mn 2 O 3 -Ag nanofibers. The Mn 2 O 3 -Ag nanofibers were used as facilitator electron transfer between Hb and glassy-carbon-modified electrode. The Mn 2 O 3 -Ag nanofibers are studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The hemoglobin showed a quasireversible electrochemical redox behavior with a formal potential of −49 mV (versus Ag/AgCl) in 0.1 M potassium phosphate buffer solution at pH 7.0. The designed biosensor possesses good stability and reproducibility and achieves 95% of the steady-state current in less than five seconds.