Separation and enrichment of flavonoids from orange peel using magnetic nanoparticles

Abstract

In present study the Fe3O4 magnetic nanoparticles (MNPs) was acted as adsorption carrier to separate and concentrate flavonoids from the root extractive of orange peel. It involved synthesizing the Fe 3O4 magnetic nanoparticle and extracting the flavonoids from orange peel. After magnetic separation and desorption process, the eluent was injected for HPLC analysis. Some parameters influenced extraction efficiency of flavonoids, including amount of Fe3O4 magnetic nanoparticles, shaking time and desorption condition were investigated. Under the optimal conditions, experimental result showed that the extraction efficiency of neohesperidin was higher than others flavonoids and the desorption ratio of neohesperidin is 89.3 %. This study demonstrated that the developed methods for separation and enrichment flavonoids was simple, lower cost and environmental friendly. Therefore, the proposed method had a potential for further application.