Clinical proteomics: Liquid chromatography–Mass spectrometry (LC–MS) purification systems

Abstract

Liquid chromatography–mass spectrometry (LC–MS) has become a routine powerful technology in clinical proteomic studies for protein identification, protein characterization and the discovery of biomarkers. In this chapter, we describe two protocol methods to analyze clinical patient samples using a resin based depletion column followed by either protein In-gel enzymatic digestion or protein in-solution enzymatic digestion and then analysis by one-dimensional reverse-phase chromatography or two-dimensional strong cation exchange (SCX)—reverse-phase chromatography (RPC).