Weak or transient protein-protein interactions (PPIs) are involved in a manifold of cellular processes in all living organisms, including plants. However, many of these interactions may remain undiscovered by co-immunoprecipitation (Co-IP) approaches due to their low binding affinities or transitory nature. Enzyme-mediated proximity-dependent in vivo biotin labeling can be a powerful strategy to efficiently capture weak and transient PPIs and has been successfully applied in different model angiosperm species. Here, we provide an optimized and robust protocol for biotin ligase-mediated proximity labeling for interactome mapping in the model liverwort Marchantia polymorpha.
CITATION STYLE
Melkonian, K., Stolze, S. C., Harzen, A., & Nakagami, H. (2023). Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha. In Methods in Molecular Biology (Vol. 2581, pp. 295–308). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2784-6_21
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