Nanoelectrokinetic bufferchannel-less radial preconcentrator and online extractor by tunable ion depletion layer

Abstract

Among various preconcentration strategies using nanofluidic platforms, a nanoscale electrokinetic phenomenon called ion concentration polarization (ICP) has been extensively utilized due to several advantages such as high preconcentration factor and no need of complex buffer exchange process. However, conventional ICP preconcentrator had difficulties in the recovery of preconcentrated sample and complicated buffer channels. To overcome these, bufferchannel-less radial micro/nanofluidic preconcentrator was developed in this work. Radially arranged microchannel can maximize the micro/nano membrane interface so that the samples were preconcentrated from each microchannel. All of preconcentrated plugs moved toward the center pipette tip and can be easily collected by just pulling out the tip installed at the center reservoir. For a simple and cost-effective fabrication, a commercial printer was used to print the nanoporous membrane as "Nafion-junction device." Various analytes such as polystyrene particle, fluorescent dye, and dsDNA were preconcentrated and extracted with the recovery ratio of 85.5%, 79.0%, and 51.3%, respectively. Furthermore, we used a super inkjet printer to print the silver electrode instead of nanoporous membrane to preconcentrate either type of charged analytes as "printed-electrode device." A Faradaic reaction was used as the main mechanism, and we successfully demonstrated the preconcentration of either negatively or positively charged analytes. The presented bufferchannel-less radial preconcentrator would be utilized as a practical and handy platform for analyzing low-abundant molecules.