Micro-PIV as a research tool for in vivo studies of vascular remodeling

Abstract

Blood flow plays a crucial role in the development and adaptation of vascular networks. In this study, we present a non-intrusive methodology for detailed measurement of the relevant haemodynamic parameters in vivo. The technique is based on two steps: first, the three-dimensional flow velocity field is determined using scanning microscopic Particle Image Velocimetry. Subsequently, this data is used to derive parameters such as the wall shear stress, blood vessel geometry, local perfusion rates, etc. The technique is demonstrated in the vitelline network of a chicken embryo, but is applicable to any system that has optical access. © 2009 Springer Berlin Heidelberg.