Fabrication of antibacterial and hemostatic electrospun PVA nanofibers for wound healing

Abstract

Health care professionals have always had problems treating and healing wounds. Today, many of these problems have been resolved with the use of advanced wound dressings. The main aim of this work was to fabricate the wound dressings using the electrospinning method blending Tranexamic acid (TXA) with Polyvinyl alcohol (PVA) to investigate the blood coagulation properties. Also, the blending of Ceftriaxone (CTX) with Polyvinyl alcohol (PVA) was done to consider antibacterial properties. The effects of drug concentration changes on these properties were also considered. Morphological results demonstrated that nanofiber was produced uniformly and without beads. SEM image indicated that the average diameter of the nanofiber of PVA with 10 and 20 mg/ml of TXA was 110, 124 nm, respectively and the average diameter of PVA nanofibers was with 0.1, 1, 8 µg/ml of CTX was 258, 273 and 379 nm, respectively. The antibacterial properties of Gram-negative (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus) were studied and the results demonstrated that the antibacterial properties increased with increasing MIC. In PVA/CTX dressing with MIC: 8 μg/ml reached 100%. Both PVA-TXA (10 mg/ml) and PVA-TXA (20 mg/ml) dressings demonstrated the acceptable ability of blood coagulation. PVA-TXA (20 mg/ml) with an average absorption of 0.031 had higher blood coagulation ability.