Using Drosophila actin-binding protein Dunc-115 as model system, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single-cell-level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.
CITATION STYLE
He, Q., & Roblodowski, C. (2016). Functional analysis of actin-binding proteins in the central nervous system of drosophila. In Methods in Molecular Biology (Vol. 1365, pp. 349–355). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3124-8_20
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