Quorum sensing in gram-positive bacteria: Assay protocols for staphylococcal agr and enterococcal fsr systems

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Abstract

A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.

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Shojima, A., & Nakayama, J. (2014). Quorum sensing in gram-positive bacteria: Assay protocols for staphylococcal agr and enterococcal fsr systems. Methods in Molecular Biology, 1147, 33–41. https://doi.org/10.1007/978-1-4939-0467-9_3

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