Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for measurement of isotopic labeling, which is required for generating comprehensive flux maps using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in a wide range of biochemical species, including proteinogenic amino acids, free organic and amino acids, sugar phosphates, lipids, starch-glucose, and RNA-ribose. We describe the steps of sample preparation, MS analysis, and data handling required to obtain high-quality isotope labeling measurements that are applicable to MFA. By selecting target analytes that maximize identifiability of the key fluxes of interest, MS measurements of isotope labeling can provide a powerful platform for assessing metabolic fluxes in complex biochemical networks. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Young, J. D., Allen, D. K., & Morgan, J. A. (2014). Isotopomer measurement techniques in metabolic flux analysis II: Mass spectrometry. Methods in Molecular Biology, 1083, 85–108. https://doi.org/10.1007/978-1-62703-661-0_7
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