Detection of Antibiotic Resistance, Virulence Gene, and Drug Resistance Gene of Staphylococcus aureus Isolates from Bovine Mastitis

Abstract

Bovine mastitis is a serious disease associated with both high incidence and economic loss, posing a major challenge to the dairy industry worldwide. Staphylococcus aureus is one of the most common pathogens to cause bovine mastitis, and antimicrobial therapy plays an important role in mastitis control caused by S. aureus but has become less effective due to widespread drug resistance. The purpose of this study was to detect antibiotic resistance, drug resistance gene, and virulence gene of S. aureus strains, which would be helpful to mastitis control. Antimicrobial therapy plays an important role in mastitis control caused by Staphylococcus aureus but has become less effective due to widespread drug resistance. The purpose of this study was to detect antibiotic resistance, drug resistance gene, and virulence gene of S. aureus strains. In this study, 2,962 milk samples were collected from 43 dairy farms located in 16 provinces of China and cultured for isolation of S. aureus . Antibiotic resistance, capsular polysaccharide, spa typing, virulence genes, and drug resistance genes of the strains were analyzed. Of 2,962 samples, 298 strains were isolated and identified as S. aureus . The strains exhibited high percentages of resistance to penicillin G (91.95%). Moreover, all strains showed resistance to more than one antimicrobial agent but were sensitive to nitrofurantoin and sulfamethoxazole/trimethoprim. The results indicate that type 8 was the dominant capsular polysaccharide serotype and t459 was the dominant spa type. The most prevalent virulence gene was clfA (98%). The resistance genes of several antibiotics were detected, among which the blaZ gene (92.95%) was the highest. In conclusion, we present the antimicrobial resistance and virulence genes of S. aureus in this study which are of importance for mastitis control. IMPORTANCE Bovine mastitis is a serious disease associated with both high incidence and economic loss, posing a major challenge to the dairy industry worldwide. Staphylococcus aureus is one of the most common pathogens to cause bovine mastitis, and antimicrobial therapy plays an important role in mastitis control caused by S. aureus but has become less effective due to widespread drug resistance. The purpose of this study was to detect antibiotic resistance, drug resistance gene, and virulence gene of S. aureus strains, which would be helpful to mastitis control.