Loop-mediated isothermal amplifi cation (lamp) for detection of phytoplasmas in the field

Abstract

Loop-mediated isothermal amplifi cation (LAMP) is a nucleic acid-based detection method with many applications. This chapter details its use for detection of phytoplasma diseases, combining a rapid 2-min DNA extraction method with real-time LAMP product detection such that the entire procedure can be undertaken and completed in the fi eld within 40 min. Furthermore, the assays include an anneal curve validation step to guard against false positives and an assay for host plant DNA to guard against false negatives.