A major focus in synthetic biology is the rational design and implementation of gene circuits to control dynamics of individual cells and, increasingly, cellular populations. Population-level control is highlighted in recent studies which attempt to design and implement synthetic ecosystems (or engineered microbial consortia). On the one hand, these engineered systems may serve as a critical technological foundation for practical applications. On the other hand, they may serve as well-defined model systems to examine biological questions of broad relevance. Here, using a synthetic predator-prey ecosystem as an example, we illustrate the basic experimental techniques involved in system implementation and characterization. By extension, these techniques are applicable to the analysis of other microbial-based synthetic or natural ecosystems. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Payne, S., Smith, R. P., & You, L. (2012). Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem. Methods in Molecular Biology, 813, 315–330. https://doi.org/10.1007/978-1-61779-412-4_19
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