Cold storage effects on fitness of the whitefly parasitoids encarsia sophia and eretmocerus hayati

Abstract

Successful biological control of the whitefly Bemisia tabaci involves the mass rearing of biocontrol agents in large numbers for field release. Cold storage of the biocontrol agents is often necessary to provide a sufficient number of biocontrol agents during an eventual pest outbreak. In this study, the fitness of two whitefly parasitoids Encarsia sophia Girault and Dodd (Hymenoptera: Aphelinidae) and Eretmocerus hayati Zolnerowich and Rose (Hymenoptera: Aphelinidae) was evaluated under fluctuating cold storage temperatures. The emergence rate of old pupae of either species was not affected when stored at 12, 10, 8 and 6◦C for 1 week. Cold storage had no effect on the longevity of the emerging adult En. sophia except young pupae stored at 4◦C, while Er. hayati was negatively affected after 2 weeks of storage time at all temperatures. Parasitism by adults emerging from older pupae stored at 12◦C for 1 week was equivalent to the control. Combined with the results for the emergence time, we suggest that the old pupal stage of En. sophia and Er. hayati could be stored at 12 and 10◦C, respectively (transferred every 22 h to 26 ± 1◦C for 2 h), for 1 week, with no or little adverse effect.