Small interfering RNAs (siRNAs) have emerged as a powerful technique for sequence-specific gene silencing in a wide variety of organisms. However, it has been shown that in vitro transcribed siRNA can activate innate immunity through RIG-1 helicase, a cytosolic sensor of viral RNA. We recently demonstrated that the activation of RIG-1 by siRNA-bearing 5'-triphosphate can be used to enhance dendritic cell maturation and function. Indeed, immature DCs that had been transfected with siRNA-bearing 5'-triphosphate activated T cells, indicating that, even in the absence of external stimuli such as TNF-alpha, those DCs were sufficiently mature to initiate T cell activation. Also, a recent study has shown that 5'-bearing siRNAs targeting Bcl-2 can induce apoptosis in tumour cells, further supporting the use of this strategy to combat cancer. This chapter describes the in vitro synthesis of siRNA targeting indoleamine 2,3-dioxygenase (IDO), an enzyme that plays a critical role in hindering immunity against tumours.
CITATION STYLE
Sioud, M. (2010). Modulation of dendritic cell maturation and function by siRNA-bearing 5’-triphosphate. Methods in Molecular Biology (Clifton, N.J.), 629, 395–404. https://doi.org/10.1007/978-1-60761-657-3_26
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