Analytical aspects of high performance thin layer chromatography

Abstract

High performance thin layer chromatographic method is applied for qualitative and quantitative analysis. Qualitative analysis was used for identification of drugs/chemicals, or certain toxic residuals compounds in food or other preparations, and finger printing of herbal drugs/biological matrixes. Quantitative analysis was used for the determination of drugs/metabolites in pharmaceutical preparations or biological matrixes, impurities, residual toxic compounds, drug stability testing, and quality control purposes. For obtaining reliable analysis results, the (high performance) thin layer chromatographic method should be validated first, before using it as a routine quality control tools. The validation parameters, which should be evaluated, are stability of the analyte, specificity/selectivity, linearity, accuracy, precision, range, detection limit/decision limit, quantification limit/detection capability, and robustness/ruggedness. © 2011 Springer-Verlag Berlin Heidelberg.