A glucan from active dry baker's yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure

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Abstract

The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry baker's yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry. NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1 → 3 )-linked β-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1 → 3)-β-oligoglucosides. linked to the main chaing through (1 → 6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the β-D-configuration.

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Zlatković, D., Jakovljević, D., Zeković, D., & Vrvić, M. M. (2003). A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure. Journal of the Serbian Chemical Society, 68(11), 805–809. https://doi.org/10.2298/JSC0311805Z

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