Tricine-SDS-PAGE

Abstract

Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is an efficient way of separating low-molecular-mass proteins. However, the standard system is quite complicated and specifically may not be useful when the separated proteins require to be recovered from the gel for quantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low-percentage gels which have high compatibility with modern detectors such as UV and inductively coupled plasma mass spectrometry (ICP-MS). © 2012 Springer Science+Business Media, LLC.