Systematic optimization of interface interactions increases the thermostability of a multimeric enzyme

Abstract

Stapling subunits: The limited stability of multimers (see picture) is often associated with subunit dissociation. A novel procedure was applied on the basis of crystal structure analysis, sequence alignment, and single-site saturation mutagenesis to systematically identify residues in the inter-subunit interface suitable for increasing stability. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.