A New Filter Assay in the Study of Lysyl-tRNA Synthetase from Bacillus stearothermophilus

Abstract

A glass microfiber filter was found useful for detection of the complex of lysyl-tRNA synthetase from Bacillus stearothermophilus with lysyladenylate. Detected c.p.m. values of the 3H-labeled complex trapped by the glass microfiber filter was about 2-fold higher than those by nitrocellulose filter and about 3-fold higher than those by DEAE-cellulose filter because of its higher counting efficiency. The use of the glass fiber filter is more suitable for the lysyl-tRNA synthetase assay than the use of either nitrocellulose or DEAE-cellulose filters, which were used for other aminoacyl-tRNA synthetases. Effects of pH on the filter assay of lysyl-tRNA synthetase activity using the glass microfiber filter were also examined. © 1997, Taylor & Francis Group, LLC. All rights reserved.