Homogenates of rat adipocytes and plasma membranes thereof were shown by radioimmunoassay to contain immunoreactive glandular kallikrein. On the basis of the hydrolysis of D-Val-Leu-Arg-p-nitroanilide, the kallikrein-like enzyme associated with the plasma membranes was found not to be stimulated by prior incubation with melittin or phospholipase A2. However, pre-incubation of the membrane preparation with trypsin did increase the activity of the enzyme. Furthermore, activation could also be achieved by incubating the plasma membranes with insulin at a dose that stimulated glucose uptake into intact adipocytes. On the other hand, incubation with insulin at a dose that did not increase glucose uptake into rat adipocytes was ineffective in activating the kallikrein-like enzyme.
CITATION STYLE
Mulholland, F., Ashford, A., & Bailey, G. S. (1986). Initial observations of a kallikrein-like enzyme associated with the plasma membranes of rat adipocytes. Advances in Experimental Medicine and Biology, 198 Pt A, 41–46. https://doi.org/10.1007/978-1-4684-5143-6_6
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