Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.
CITATION STYLE
Qi, Y., & Mi, S. (2010). Purification of Arabidopsis argonaute complexes and associated small RNAs. Methods in Molecular Biology (Clifton, N.J.), 592, 243–254. https://doi.org/10.1007/978-1-60327-005-2_16
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