Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated genes. Further expression profiling experiments showed how these genes are regulated by the VpsR and HapR transcription factors, which, respectively, positively and negatively regulate production of VPS El Tor, a rugose-associated extracellular polysaccharide. The study of mutants of rpoN and rpoS demonstrated the effects of these alternative sigma factors on phase variation-specific gene expression. Bioinforroatics analysis of these expression data shows that 'rugosity' and 'smoothness' are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism.
CITATION STYLE
Yildiz, F. H., Liu, X. S., Heydorn, A., & Schoolnik, G. K. (2004). Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant. Molecular Microbiology, 53(2), 497–515. https://doi.org/10.1111/j.1365-2958.2004.04154.x
Mendeley helps you to discover research relevant for your work.