Neurosphere assay is a common and robust method for identification of neural stem/progenitor cells, but obtaining large numbers of live single cells from dissociated neurospheres is difficult using nonenzymatic methods. Here, we present an enzyme-free method for high-efficiency neurosphere dissociation into single cells using microfluidic device technology. This method allows single cell dissociation of DC115 and KT98 cells with high cell viabilities (80–85%), single-cell yield (91–95%), and recovery (75–93%).
CITATION STYLE
Lin, C. H., Chang, H. C., & Hsu, C. H. (2016). Enzyme-free dissociation of neurospheres by a microfluidic chip-based method. In Methods in Molecular Biology (Vol. 1516, pp. 289–297). Humana Press Inc. https://doi.org/10.1007/7651_2016_348
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