In this study metabolic footprinting was applied to evaluate the metabolic consequences of protein overproduction at slow growth conditions (mu = 0.1 h(-1)). The extracellular metabolites detected by gas chromatography-mass spectrometry characterized the metabolic footprints before and after the induction of the recombinant protein production (i.e. pre- and post-induction phases). Metabolic footprinting enabled the discrimination between the two growth phases and exposed significant alterations in the extracellular milieu during the recombinant process.
CITATION STYLE
Carneiro, S., Villas-Bôas, S. G., Rocha, I., & Ferreira, E. C. (2010). Applying a Metabolic Footprinting Approach to Characterize the Impact of the Recombinant Protein Production in Escherichia coli (pp. 193–200). https://doi.org/10.1007/978-3-642-13214-8_25
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