Nitric oxide preserves the glomerular protein permeability barrier by antagonizing superoxide

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Abstract

Background. The interaction of nitric oxide with superoxide (O 2-) is a major O2- scavenging mechanism that can minimize O2--mediated oxidative stress. Glomeruli produce both nitric oxide and O2- and generation of both radicals is increased in various forms of glomerular disease. O2- increases glomerular capillary permeability to albumin (Palb). The present studies tested the hypothesis that nitric oxide opposes this effect, thereby preserving the glomerular protein permeability barrier. Methods. Palb was determined in isolated rat glomeruli by measuring the change in glomerular volume in response to an experimental oncotic gradient. Changes in Palb in response to O 2- generated by tumor necrosis factor-alpha (TNF-α) or xanthine/xanthine oxidase (X/XO) was assessed under conditions of nitric oxide depletion and repletion. Results. Incubation of rat glomeruli with the nitric oxide synthase (NOS) inhibitor L-NG-monomethylarginine (L-NMMA) increased Palb. This effect was reversed by the nitric oxide donor diethylenetriamine NONOate (DETA-NONOate) and by the Superoxide dismutase (SOD) mimetic Tempol. O2- generated after incubation with TNF-α or X/XO increased Palb. This effect was blocked by DETA-NONOate. Conclusion. We demonstrate that nitric oxide protects the glomerular filtration barrier from injury caused by O2- and suggest that inhibition of nitric oxide synthesis could enhance O 2--mediated oxidative injury under pathologic conditions. © 2005 by the International Society of Nephrology.

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APA

Sharma, M., McCarthy, E. T., Savin, V. J., & Lianos, E. A. (2005). Nitric oxide preserves the glomerular protein permeability barrier by antagonizing superoxide. Kidney International, 68(6), 2735–2744. https://doi.org/10.1111/j.1523-1755.2005.00744.x

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