It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.
CITATION STYLE
Tripathi, V., Fei, J., Ha, T., & Prasanth, K. V. (2015). RNA fluorescence in situ hybridization in cultured mammalian cells. Methods in Molecular Biology, 1206, 123–136. https://doi.org/10.1007/978-1-4939-1369-5_11
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