Serum-free suspension cultures are preferably required for recombinant protein production due to its readiness in upstream/downstream processing and scale-up, therefore increasing process productivity and competitiveness. This type of culture replaces traditional cell culturing as the presence of animal-derived components may introduce lot-a-lot variability and adventitious pathogens to the process. However, adapting cells to serum-free conditions is challenging, time-consuming, and cell line and medium dependent. In this chapter, we present different approaches that can be used to adapt mammalian cell lines from an anchorage-dependent serum supplemented culture to a suspension serum-free culture.
CITATION STYLE
Caron, A. L., Biaggio, R. T., & Swiech, K. (2018). Strategies to suspension serum-free adaptation of mammalian cell lines for recombinant glycoprotein production. In Methods in Molecular Biology (Vol. 1674, pp. 75–85). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7312-5_6
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