Traditional differential expression tools are limited to detecting changes in overall expression, and fail to uncover the rich information provided by single-cell level data sets. We present a Bayesian hierarchical model that builds upon BASiCS to study changes that lie beyond comparisons of means, incorporating built-in normalization and quantifying technical artifacts by borrowing information from spike-in genes. Using a probabilistic approach, we highlight genes undergoing changes in cell-to-cell heterogeneity but whose overall expression remains unchanged. Control experiments validate our method's performance and a case study suggests that novel biological insights can be revealed. Our method is implemented in R and available at https://github.com/catavallejos/BASiCS.
CITATION STYLE
Vallejos, C. A., Richardson, S., & Marioni, J. C. (2016). Beyond comparisons of means: Understanding changes in gene expression at the single-cell level. Genome Biology, 17(1). https://doi.org/10.1186/s13059-016-0930-3
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