A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break. A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a double-strand break, the repair of which is also quantifiable.
CITATION STYLE
Pierce, A. J., & Jasin, M. (2005). Measuring recombination proficiency in mouse embryonic stem cells. Methods in Molecular Biology (Clifton, N.J.), 291, 373–384. https://doi.org/10.1385/1-59259-840-4:373
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