Establishing an in Vitro Model to Assess the Toxicity of 6PPD-Quinone and Other Tire Wear Transformation Products

9Citations
Citations of this article
33Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The tire wear transformation product 6PPD-quinone (6PPDQ) has been implicated as the causative factor for broad scale mortality events for coho salmon in the Pacific Northwest. Highly variable sensitivity to 6PPDQ in closely related salmonids complicates efforts to evaluate the broader toxicological impacts to aquatic ecosystems. Our goals were to (1) validate the large range of in vivo species sensitivities reported for coho, Chinook, and sockeye salmon and (2) develop an in vitro platform for assessing 6PPDQ toxicity. In vivo studies confirmed the acute sensitivity of juvenile coho (12 h LC50= 80.4 ng/L) and demonstrated that sockeye salmon were not vulnerable to mortality. Chinook salmon were sensitive to 6PPDQ mortality at initial concentrations >25 μg/L, ∼10-fold greater than reported environmental measurements. In vitro, the coho salmon cell line CSE-119 was acutely sensitive to 6PPDQ (metabolic EC50= 7.9 μg/L, cytotoxicity EC50= 6.1 μg/L). Analogous Chinook (CHSE-214) and sockeye salmon (SSE-5) cell lines were nonresponsive in both assays, and rainbow trout RTG-2 cells began showing metabolic effects at 68 μg/L (EC5). Recreation of species-specific 6PPDQ sensitivity in vitro implicates conserved modes of action in CSE-119 that could be utilized for mechanistic studies of 6PPDQ toxicity and screening of other PPD transformation products.

Cite

CITATION STYLE

APA

Greer, J. B., Dalsky, E. M., Lane, R. F., & Hansen, J. D. (2023). Establishing an in Vitro Model to Assess the Toxicity of 6PPD-Quinone and Other Tire Wear Transformation Products. Environmental Science and Technology Letters, 10(6), 533–537. https://doi.org/10.1021/acs.estlett.3c00196

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free