The potential of the mushroom log waste (MLW) and Azotobactervinelandii to improve hydrocarbon biodegradation for rehabilitation of petroleum contaminated soil

Abstract

Bioremediation is the biological technology for removing toxic and complex pollutants in soil and water system by degrading or transforming the pollutants becoming harmless compounds. The research focused on determining the Mushroom log waste (MLW) and Azotobactervinelandii effect to improve the rate and efficiency of petroleum degradation in soil and assessment of functional microbial existing in that soil system. The randomized block design (RBD) was used with six treatments and three replications consisted of: Control; A. vinelandii; Petrophylic; Mushroom log waste (MLW); A. vinelandii + MLW; Petrophylic + MLW. The result showed that A. vinelandii treatment accelerated the hydrocarbon degradationrate from 0.007811 day -1 to 0.011038 day -1 .While the hydrocarbons degradation rate by MLW treatmentreached 0.026354 day -1 , with a biodegradation efficiency of 81.716 %. The MLW also enhancedbacterial petrophylicgrowth rate from 0.263029 day -1 to 0.357115 day -1 , while Azotobacterspp.growth rate enhanced from 0.057886 day -1 to 0.250719 day -1 . The MLW has potential used for supporting soil rehabilitation of petroleum waste, also increased existing of Azotobacter spp. population.