Tetracycline-controlled transcription in eukaryotes: Novel transactivators with graded transactivation potential

295Citations
Citations of this article
245Readers
Mendeley users who have this article in their library.

Abstract

Several tetracycline-controlled transactivators (tTA) were generated which differ in their activation potential by > 3 orders of magnitude. The transactivators are fusions between the Tet repressor and minimal transcriptional activation domains derived from Herpes simplex virus protein 16 (VP16). By reducing the VP16 moiety of the previously described tTA to 12 amino acids, potential targets for interactions with various cellular transcription factors were eliminated, as were potential epitopes which may elicit a cellular immune response. When compared with the originally described tTA, these new transactivators are tolerated at higher intracellular concentrations. This will facilitate establishment of tet regulatory systems under a variety of conditions, but particularly when cell type-restricted tetracycline-controlled gene expression is to be achieved in transgenic organisms via homologous recombination.

References Powered by Scopus

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

232748Citations
N/AReaders
Get full text

Tight control of gene expression in mammalian cells by tetracycline- responsive promoters

4406Citations
N/AReaders
Get full text

Transcriptional activation by tetracyclines in mammalian cells

2135Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells

951Citations
N/AReaders
Get full text

The TetR family of transcriptional repressors

897Citations
N/AReaders
Get full text

Synthetic biology: New engineering rules for an emerging discipline

867Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Baron, U., Gossen, M., & Bujard, H. (1997). Tetracycline-controlled transcription in eukaryotes: Novel transactivators with graded transactivation potential. Nucleic Acids Research, 25(14), 2723–2729. https://doi.org/10.1093/nar/25.14.2723

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 100

53%

Researcher 54

28%

Professor / Associate Prof. 31

16%

Lecturer / Post doc 5

3%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 103

56%

Biochemistry, Genetics and Molecular Bi... 56

30%

Medicine and Dentistry 15

8%

Neuroscience 10

5%

Save time finding and organizing research with Mendeley

Sign up for free