Separation of Target Microbe in Microchip by Flow Balancing and Visually Guided Laser Tele-Manipulator

Abstract

We developed a new system for random separation of a microobject, such as a microbe, in the micro fluidic devise under the microscope by integrating the laser-trapping force and dielectrophoretic (DEP) force. An arbitrary selected single microbe was isolated in a microchannel, even though there are a large number of microbes in solution. To realize an efficient separation system, we proposed a new separation cell and made it by microfabrication. The flow speed in the micro channel is adjusted and balanced to realize high speed and high purity extraction of the target microbe. The target is trapped by the laser and transported by the vision based control system to be taken out from the extraction port.