Pulsed stable isotope labeling by amino acids in cell culture (pSILAC) comprises a variation of the classical SILAC proteomic methodology that enables the identification of short-term proteomic responses such as those elicited by micro RNAs (miRNAs). Here, we describe a detailed pSILAC protocol for global identification and quantification of protein translation alterations induced by a miRNA using 3T3-L1 preadipocytes as a model system.
CITATION STYLE
Duque-Guimarães, D. E., De Almeida-Faria, J., Ong, T. P., & Ozanne, S. E. (2017). Pulsed SILAC as a approach for miRNA targets identification in cell culture. In Methods in Molecular Biology (Vol. 1546, pp. 149–159). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6730-8_11
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