Mitochondrial cytochrome-b, cytochrome-c and d-loop region based phylogenetic and diversity analysis in blackbuck (Antilope cervicapra)

Abstract

The present study was designed to find diversity analysis of Antilope cervicapra family in Pakistan. Fecal samples of Antilope cervicapra were collected from their different habitats of Pakistan. Fecal DNA was extracted and Polymerase Chain Reaction (PCR) was performed. Sequencing was performed by Big DyeTM Terminator method. Diversity and phylogenetic analysis was performed by different Bioinformatics tools. Less genetic variability was observed within Antilope cervicapra population through Multi-Dimensional Scaling (MDS). However, significant genetic variation was observed among other species and Antilope cervicapra. Phylogenetic analysis revealed distinct clade of this specie with respect to other species of deer. This is the first report from Pakistan that could help for designing effective strategy in future conservation practices of deer species.