Molecular diversity of methanogen in moderate temperature anaerobic fermentation process of water hyacinth

Abstract

It is to collect the biogas slurry in the moderate temperature anaerobic fermentation process of water hyacinth during the peak biogas generation period for extracting total genomic DNA, amplify 16S rRNA gene in total genome with 16S rDNA specific primer of methanogen, establish 16S rDNA library and analyze 16S rDNA in the library with RFLP method. The results indicated that some 1.2 Kb of target bands may be obtained by amplifying the extracted genome with 16S rDNA specific primer of methanogen; HaeIII was used to conduct restriction enzyme analysis about 16S rDNA fragments and through comparative statistics of its restriction enzyme mapping, we have obtained 23 kinds of RFLP.