Force-velocity relationship during isometric and isotonic fatiguing contractions

Abstract

Fatiguing contractions change the force-velocity relationship, but assessment of this relationship in fatigue has usually been obtained after isometric contractions. We studied fatigue caused by isometric or isotonic contractions, by assessment of the forcevelocity relationship while the contractions maintaining fatigue were continued. This approach allowed determination of the force-velocity relationship during a steady condition of fatigue. We used the in situ rat medial gastrocnemius muscle, a physiologically relevant preparation. Intermittent (1/s) stimulation at 170 Hz for 100 ms resulted in decreased isometric force to ∼35% of initial or decreased peak velocity of shortening in dynamic contractions to ∼45% of initial. Dynamic contractions resulted in a transient initial increase in velocity, followed by a rapid decline until a reasonably steady level was maintained. Data were fit to the classic Hill equation for determination of the force-velocity relationship. Isometric and dynamic contractions resulted in similar decreases in maximal isometric force and peak power. Only Vmax was different between the types of contraction (P < 0.005) with greater decrease in Vmax during isotonic contractions to 171.7 ± 7.3 mm/s than during isometric contractions to 208.8 mm/s. Curvature indicated by a/Po (constants from fit to Hill equation) changed from 0.45 ± 0.04 to 0.71 ± 0.11 during isometric contractions and from 0.51 ± 0.04 to 0.85 ± 0.18 during isotonic contractions. Recovery was incomplete 45 min after stopping the intermittent contractions. At this time, recovery of low-frequency isometric force was substantially less after isometric contractions, implicating force during intermittent contractions as a determining factor with this measure of fatigue.