Diagnosis of Ramularia collo-cygni and Rhynchosporium spp. in Barley

Abstract

Ramularia leaf spot and Rhynchosporium leaf scald are two of the major diseases of barley crops in cooler temperate countries. The methods below are aimed at the identification and quantification of fungal DNA in leaf samples but can also be used for pathogen detection from seed or DNA extracted from environmental samplers. The methods describe in detail two individual quantitative PCR tests. The successful multiplexing of assays will lead to faster throughput of samples.