Early preimplantation embryos are precious and scarce samples that contain limited numbers of cells, which can be problematic for quantitative gene expression analyses. Nonetheless, low-input genome-wide techniques coupled with cDNA amplification steps have become a gold standard for RNA profiling of as minimal as a single blastomere. Here, we describe a single-cell/single-embryo RNA sequencing (RNA-seq) method, from embryo collection to sample validation steps prior to DNA library preparation and sequencing. Key quality controls and external Spike-In normalization approaches are also detailed.
CITATION STYLE
Pérez-Palacios, R., Fauque, P., Teissandier, A., & Bourc’his, D. (2021). Deciphering the early mouse embryo transcriptome by low-input RNA-Seq. In Methods in Molecular Biology (Vol. 2214, pp. 189–205). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0958-3_13
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