SDS-PAGE immunoblot analysis

Abstract

Immunoblotting is a core method in molecular neuroscience to detect and/or quantify single proteins in a complex mixture. The whole procedure covers the solubilization of proteins, their separation by mass on an SDS-polyacrylamide gel, the electrophoretic transfer to a membrane, and the immunodetection of the target proteins by using specific antibodies. Here we provide protocols for SDS-PAGE followed by electroblotting combined with immunostaining. Useful hints are included for method optimization. © 2011 Springer Science+Business Media, LLC.