Fast and precise heating by microwave irradiation during solid-phase peptide synthesis (SPPS) can reduce reaction times as well as provide better purities and greater yields for the synthesis of difficult peptides. Microwave- assisted SPPS has proven to be a useful and reliable tool for the synthesis of peptides as well as small proteins. It is particularly well suited for sequences with a high propensity to form β-sheet-type structures and for sterically difficult couplings. In this protocol, conditions and detailed procedures are described for performing microwave-assisted SPPS using the Syro Wave™. Here we describe the synthesis of two difficult peptide sequences: the first is derived from the C-terminus of the MuLV CTL epitope, the second is a de novo designed peptide with a C-terminal alkyne. © Springer Science+Business Media, New York 2013.
CITATION STYLE
Malik, L., & Jensen, K. J. (2013). Microwave-assisted solid-phase peptide synthesis using the biotage Syro WaveTM. Methods in Molecular Biology, 1047, 225–234. https://doi.org/10.1007/978-1-62703-544-6_16
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