Manipulation of alternative splicing is a unique way of regulating gene expression. Here, a detailed protocol is presented for modification of HER2 pre-mRNA alternative splicing. A 20-mer splice switching oligonucleotide (SSO) targeting exon 15 in HER2 pre-mRNA induced skipping of exon 15, resulted in significant downregulation of full-length HER2 mRNA and protein expression in HER2-overexpressing breast cancer cell line SK-BR-3. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Wan, J. (2012). Antisense-mediated exon skipping to shift alternative splicing to treat cancer. Methods in Molecular Biology, 867, 201–208. https://doi.org/10.1007/978-1-61779-767-5_13
Mendeley helps you to discover research relevant for your work.