Development of novel thioguanosine analogs with the ability to specifically modify cytidine.

Abstract

Site-specific modification of nucleic acid is of great significance in the machinery of gene expression. Specific modification of nucleic acids by an oligonucleotide incorporating a S-vinyl thioguanosine analog, has great potential as a useful tool. The specific transfer of the vinyl derivative to the amino group of dC at the target site of the complementary ODN has been demonstrated. This is an innovative method for the efficient and selective modification of the target cytidine in DNA.