Detection of urinary metabolomics before and after pringle maneuver-induced liver ischemia and reperfusion injury in rats using gas chromatography-mass spectrometry

Abstract

Background. Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems. Objective. To detect urinarymetabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringlemaneuver using gas chromatographymass spectrometry (GC-MS). Methods. Male Sprague-Dawley rats (N = 80) were randomly divided into 4 groups (n = 20/group): sham operation, day 1, day 3, and day 5. Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver. Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed. GC-MS was used to detect urinary metabolomics. Results. Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated (P < 0.01) and then decreased and reached close to normal levels at day 5. GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination. Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (P < 0.001). Principle component analysis showed that 7metabolites distinguished the day 1 and day 3 groups fromthe shamgroup. Conclusions. Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury. Copyright © 2013 Peter Kruzliak et al.