Application of Streptomyces as a probiotic in the laboratory culture of Penaeus monodon (Fabricius)

Abstract

Probiotic supplementation of live microorganisms in aquaculture aids in preventing disease, thereby increasing production and decreasing economic loss. In the present study, Streptomyces cells were incorporated in Penaeus monodon (Fabricius) laboratory culture for 25 days. Streptomyces was inoculated into the feed in different concentrations (0, 2.5, 5.0, 7.5, and 10.0 g/kg feed) on days 1, 10, and 20. Growth was monitored on days 5, 15, and 25. Experimental culture tanks provided with Streptomyces had better water quality parameters than the control tank. At the cell concentration of 10 g, the pH was 7.9, ammonia 0.00067 ppm, nitrate 0.00285 ppm, phosphate 0.00224 ppm, silicate 0.00836 ppm, total heterotrophic bacteria 3.279 × 105 CFU/ml, and total Vibrio 0.2 × 102 CFU/ml. Growth increased as the Streptomyces cell concentration increased. At 10 g concentration, growth in lengh and weight was 15.79% and 57.97%, respectively, and the interval between molts was 12.5 days.