This protocol specifies an accurate and sensitive method for the determination of T-2 and HT-2 toxins content in oats and oat-based foods using liquid chromatography with tandem mass spectrometry detection. T-2 and HT-2 toxins are extracted from the test material with a mixture of acetonitrile and water. The filtered extract is dried, reconstituted with a mixture of methanol and water, then purified on a polymeric solid-phase extraction cartridge. Toxins are finally eluted from the column with methanol and quantified by reversed phase high performance liquid chromatography with tandem mass spectrometry detection.
CITATION STYLE
Lattanzio, V. M. T., & Pascale, M. (2017). Determination of T-2 and HT-2 toxins in oats and oat-based breakfast cereals by liquid-chromatography tandem mass spectrometry. In Methods in Molecular Biology (Vol. 1536, pp. 127–136). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6682-0_9
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