Claustrum: a case for directional, excitatory, intrinsic connectivity in the rat

Abstract

Claustrum, a gray matter structure that underlies the neocortex, is reciprocally connected with many neocortical and limbic cortical areas. This connectivity positions claustrum ideally for the integration or coordination of widespread cortical activity. In anatomical studies using multiple planes of section, claustrum has distinct subregions based on latexin immunohistochemistry, and an approximately rostro-caudal alignment of fusiform cells supporting a laminar intrinsic organization. Physiological studies of claustral connectivity in disinhibited brain slices demonstrate (1) intrinsic connectivity sufficient to generate spontaneous synchronized burst discharges, (2) activity spread within the oblique laminae that contained the principal cellular axis, and (3) segregation of activity as evidenced by the absence of spread within coronal planes. Activity spread depended on glutamatergic synaptic transmission, and activity restrictions did not depend on inhibitory circuits. We conclude that the claustrum has an intrinsic excitatory connectivity that is constrained in approximately rostro-caudal laminae, with minimal cross-communication between laminae. Further, claustrum has the intrinsic capability of generating synchronized population activity and facilitating its spread within laminae, a feature that may contribute to seizure generation and spread.