First detailed reconstruction of the karyotype of Trachypithecus cristatus (Mammalia: Cercopithecidae)

Abstract

Background: The chromosomal homologies of human (Homo sapiens = HSA) and silvered leaf monkey (Trachypithecus cristatus = TCR) have been previously studied by classical chromosome staining and by fluorescence in situ hybridization (FISH) applying chromosome-specific DNA probes of all human chromosomes in the 1980s and 1990s, respectively. Results: However, as the resolution of these techniques is limited we used multicolor banding (MCB) at an ∼250-band level, and other selected human DNA probes to establish a detailed chromosomal map of TCR. Therefore it was possible to precisely determine evolutionary conserved breakpoints, orientation of segments and distribution of specific regions in TCR compared to HSA. Overall, 69 evolutionary conserved breakpoints including chromosomal segments, which failed to be resolved in previous reports, were exactly identified and characterized. Conclusions: This work also represents the first molecular cytogenetic one characterizing a multiple sex chromosome system with a male karyotype 44,XY§ssub§1§esub§Y§ssub§2§esub§. The obtained results are compared to other available data for old world monkeys and drawbacks in hominoid evolution are discussed. © 2013Xiaobo et al.; licensee BioMed Central Ltd.